Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: comparative karyological analysis within Ostreidae
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2004
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Springer Science
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Reliable banding techniques are a major necessity for genetic research in oysters. In this study, we carried
out the cytogenetic characterization of four oyster species (family Ostreidae) using restriction endonuclease treatments.
Chromosomes were treated with three different restriction enzymes, stained with Giemsa, and examined for banding
patterns. The following species were studied: Crassostrea gigas (2n = 20; total number of bands with ApaI, 74; HaeIII,
61; PstI, 76), Crassostrea angulata (2n = 20; ApaI, 62; HaeIII, 61; PstI, 55) (subfamily Crassostreinae), Ostrea edulis
(2n = 20; ApaI, 82; HaeIII, 59; PstI, 66), and Ostrea conchaphila (2n = 20; ApaI, 68; HaeIII, 62; PstI, 69) (subfamily
Ostreinae). Treatment of samples with ApaI, HaeIII, and PstI produced specific banding patterns, which demonstrates
the potential of these enzymes for chromosome banding in oysters. This is of special interest, since it has been recently
shown in mammalian chromosomes that restriction enzyme banding is compatible with fluorescence in situ hybridization. This study therefore provides a fundamental step in genome mapping of oysters, since chromosome banding with
restriction enzymes facilitates physical gene mapping in these important aquaculture species. The analysis of the
banded karyotypes revealed a greater similarity within the genera of Crassostrea and Ostrea than between them
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Leitão, Alexandra; Chaves, Raquel; Santos, Sara; Guedes-Pinto, Henrique; Boudry, Pierre. Restriction enzyme digestion chromosome banding in Crassostrea and Ostrea species: Comparative karyological analysis within Ostreidae, Trabalho apresentado em 15th International Chromosome Conference, In 15th International Chromosome Conference, West London, 2004.