The behavioural and histopathological effects of ketamine/medetomidine anaesthetic combination in the brain of adult mice
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2009
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A combinação cetamina/medetomidina é frequentemente utilizada na rotina de hospitais, clínicas veterinárias e em investigação. Porém, existe grande desconhecimento relativamente ao efeito desta combinação anestésica a nível cerebral, principalmente em animais adultos. Para investigar os efeitos comportamentais e histopatológicos desta combinação anestésica no cérebro do murganho adulto, cinquenta e nove murganhos, com 10, 27 e 28 semanas de idade, foram divididos em 4 grupos diferentes (controlo, 0ket+1med, 25ket+1med, 75ket+1med). Trinta e um desses animais foram submetidos a testes comportamentais: T-maze (28h, 1 e 2 semanas após a anestesia), vertical pole e open field; e os restantes foram usados para análise histopatológica cerebral (coloração de hematoxilina-eosina). O hipocampo, cortéx retrosplenial e parietal foram analisados de modo a proceder à contagem das células com características morfológicas de apoptose. Não se verificaram diferenças na neurodegeneração induzida por diferentes doses de anestesia cetamina/medetomidina no cérebro do murganho adulto. Estas observações foram reforçadas pelos estudos comportamentais, no entanto dos resultados obtidos parece pertinente a continuação e aprofundamento dos trabalhos aqui apresentados, alguns dos quais estão já a decorrer.
The combination of ketamine / medetomidine is usually used in hospitals, veterinary clinics and research. However, little is known about this combination effects on brain in adult animals. To investigate the behavioral and histopathological effects of this anaesthetic combination in the brain of adult mice, fifty-nine mice, with 10, 27 and 28weeks old, were divided into 4 different groups (control, 0ket+1med, 25ket+1med, 75ket+1med).Thirty-one of these animals were tested in behavioral tests: T-maze (28 h, 1 and 2 weeks after anesthesia), vertical pole and open field; and the others mice were used for histopathological analysis (hematoxylin and eosin). Tissue sections of hippocampus, retrosplenial cortex and parietal cortex were examined and the cells with morphological features of apoptosis were counted. No neurodegeneration lesions were induced by different concentrations of ketamine/medetomidine anaesthesia in the brain of mice. These observations were supported by behavioural studies; however the results obtained from this set of studies point towards the need for further research in this area, some of them are already being made.
The combination of ketamine / medetomidine is usually used in hospitals, veterinary clinics and research. However, little is known about this combination effects on brain in adult animals. To investigate the behavioral and histopathological effects of this anaesthetic combination in the brain of adult mice, fifty-nine mice, with 10, 27 and 28weeks old, were divided into 4 different groups (control, 0ket+1med, 25ket+1med, 75ket+1med).Thirty-one of these animals were tested in behavioral tests: T-maze (28 h, 1 and 2 weeks after anesthesia), vertical pole and open field; and the others mice were used for histopathological analysis (hematoxylin and eosin). Tissue sections of hippocampus, retrosplenial cortex and parietal cortex were examined and the cells with morphological features of apoptosis were counted. No neurodegeneration lesions were induced by different concentrations of ketamine/medetomidine anaesthesia in the brain of mice. These observations were supported by behavioural studies; however the results obtained from this set of studies point towards the need for further research in this area, some of them are already being made.
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Dissertação de Mestrado em Medicina Veterinária
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Medicina veterinária , Anestesiologia , Animais de laboratório (murganho) , Anestésicos