Acção de anti-fúngicos e soluções anti-microbianas em isolados (Canida albicans) da cavidade oral de portadores de aparelhos ortodônticos fixos
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2009
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Candida albicans é uma levedura comensal que se encontra frequentemente na cavidade oral de indivíduos saudáveis ou doentes.
Neste sentido, estudaram-se 40 isolados (num universo de 164) de leveduras provenientes de indivíduos portadores de aparelhos ortodônticos fixos de modo a estudar a sua susceptibilidade a antifúngicos e colutórios, pela técnica de Kirby-Bauer adaptada a leveduras.
Todos os isolados se mostraram resistentes ao antifúgico metronidazol. No caso do econazol, 2,5% das estirpes são resistentes, 55% têm resistência intermédia e 42,5% são sensíveis. Ao miconazol, 35% têm resistência intermédia e 65% são sensíveis. No caso do cetoconazol, 22,5% são resistentes, 75% têm resistência intermédia e 2,5% são sensíveis. Todas as estirpes são sensíveis aos antifúngicos nistatina, anfotericina B e fluconazol. Foram detectados 9 fenotipos diferentes de acordo com os resultados da resistência/susceptibilidade aos antifúngicos.
No caso dos colutórios, foram testados 7 marcas comerciais. O que apresentou uma média de halos maior foi o Chlorohex PerioGard da Colgate (21,5 mm) e o que obteve menor média de halo foi Listerine Menta fresca (6 mm).
O óleo essencial mais eficaz contra C. albicans foi o de canela com um halo de inibição de 31,6 mm, pelo contrário, o que teve menor halo foi o óleo de tangerina com 8,3 mm.
Candida albicans is a commensal yeast comon in the oral cavity of healthy and sick people. We studied 40 C. albicans isolates, randomly choose in a universe of 164 and previously isolated from individuals with fixed orthodontic appliances, in order to study their susceptibility to antifungal agents, mouthwashes and essential oils, using the Kirby-Bauer technique adapted to yeast. All isolates showed resistance to metronidazole antifungal. In the case of econazole, 2.5% of strains were resistant, 55% had intermediate resistance and 42.5% were sensitivity. In the presence of miconazole, 35% had intermediate resistance and 65% were susceptible. In the case of ketoconazole, 22.5% were resistant, 75% had intermediate resistance and 2.5% were susceptible. All strains (100.0%) exhibited sensitivity to the antifungals nystatin, amphotericin B and fluconazole. We detected 9 different phenotypes according to the results of in vitro resistance / susceptibility to antifungal agents. In the case of mouthwashes 7 brands were tested. The average of inhibition halo diameter (mm) was higher in the Chlorohex Periogard Colgate (21.5 mm) and the lowest average halo was achieved by Listerine Fresh Mint (6.0 mm). The essential oil best effective against C. albicans was the cinnamon with an inhibition zone of 31.6 mm. The smallest halo, was obtained using the oil of tangerine (8.3 mm).
Candida albicans is a commensal yeast comon in the oral cavity of healthy and sick people. We studied 40 C. albicans isolates, randomly choose in a universe of 164 and previously isolated from individuals with fixed orthodontic appliances, in order to study their susceptibility to antifungal agents, mouthwashes and essential oils, using the Kirby-Bauer technique adapted to yeast. All isolates showed resistance to metronidazole antifungal. In the case of econazole, 2.5% of strains were resistant, 55% had intermediate resistance and 42.5% were sensitivity. In the presence of miconazole, 35% had intermediate resistance and 65% were susceptible. In the case of ketoconazole, 22.5% were resistant, 75% had intermediate resistance and 2.5% were susceptible. All strains (100.0%) exhibited sensitivity to the antifungals nystatin, amphotericin B and fluconazole. We detected 9 different phenotypes according to the results of in vitro resistance / susceptibility to antifungal agents. In the case of mouthwashes 7 brands were tested. The average of inhibition halo diameter (mm) was higher in the Chlorohex Periogard Colgate (21.5 mm) and the lowest average halo was achieved by Listerine Fresh Mint (6.0 mm). The essential oil best effective against C. albicans was the cinnamon with an inhibition zone of 31.6 mm. The smallest halo, was obtained using the oil of tangerine (8.3 mm).
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Dissertação de Mestrado em Análises Laboratoriais
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Microbiologia médica , Leveduras , Antimicótico