Antimicrobial resistance and resistance genes in Aeromonas isolated from “alheira”: a traditional portuguese fermented sausage

Fontes, M. C.
Saavedra, M. J.
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Aeromonas are autochthonous species in aquatic environment and have also been isolated from different kinds of foods, including those of animal origin, seafood and vegetables. Some species are considered important pathogens and water and foods are important sources of transmission of Aeromonas spp.. In the present work we studied the antimicrobial resistance in strains of Aeromonas isolated from “alheira”, a traditional Portuguese meat sausage naturally-fermented and smoked, and the occurrence of β-Lactamases genes among these strains. A total of 84 presumptive Aeromonas spp. isolates were obtained from 10 samples purchased in different local markets and subjected to genotyping ERIC-PCR analysis. The 20 strains showing differences in the ERIC-pattern were identified by gyrB gene sequencing and tested against 27 antimicrobial agents. β-Lactamases-encoding genes were detected by PCR using primers for detection of TEM, SHV, OXA, CTX-M, FOX and MOX variants and for the metallo-β-lactamases CphA and VIM. Tested antibiotic susceptibilities in these strains indicated a noticeable level of resistance to several groups of antibiotics. One strain of Aeromonas media harboured blaCTX-M, blaTEM and blaFOX genes. blaOXA-aer and blaFOX were detected in 12 of the 20 strains tested, and blaCphA gene in 8 strains. blaOXA-C gene was detected in 2 strains, one of Aeromonas caviae and other of Aeromonas hydrophila. blaOXA-B was presented in one strain of Aeromonas caviae. There was no evidence for the presence of blaSHV and blaVIM genes in any isolate.