Please use this identifier to cite or link to this item: http://hdl.handle.net/10348/2697
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dc.contributor.authorCoelho, Ana Cláudia-
dc.contributor.authorPinto, Maria de Lurdes-
dc.contributor.authorMiranda, Alice-
dc.contributor.authorCoelho, Adosinda-
dc.contributor.authorPires, Maria dos Anjos-
dc.contributor.authorMatos, Manuela-
dc.date.accessioned2013-09-11T15:14:21Z-
dc.date.available2013-09-11T15:14:21Z-
dc.date.issued2010-
dc.identifier.issn0975-1009-
dc.identifier.urihttp://hdl.handle.net/10348/2697-
dc.description.abstractThirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. Combination of techniques would, therefore, increase the sensitivity of diagnosis.por
dc.language.isoengpor
dc.publisherRajiv Mathurpor
dc.relation.ispartofCECAV - Centro de Ciência Animal e Veterináriapor
dc.rightsopenAccesspor
dc.subjectMycobacterium avium subsp. paratuberculosispor
dc.subjectPCRpor
dc.subjectTissuespor
dc.subjectZiehl-Neelsenpor
dc.titleComparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissues for diagnosis of Mycobacterium avium subsp. paratuberculosispor
dc.typearticlepor
degois.publication.locationNova Deli, Indiapor
degois.publication.titleIndian Journal of Experimental Biologypor
degois.publication.volume48por
dc.relation.publisherversionhttp://nopr.niscair.res.in/bitstream/123456789/10102/1/IJEB%2048%289%29%20948-950.pdfpor
dc.peerreviewedyespor
Appears in Collections:OLD - DCV - Trabalho Académico

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