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Title: Comparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissues for diagnosis of Mycobacterium avium subsp. paratuberculosis
Authors: Coelho, Ana Cláudia
Pinto, Maria de Lurdes
Miranda, Alice
Coelho, Adosinda
Pires, Maria dos Anjos
Matos, Manuela
Keywords: Mycobacterium avium subsp. paratuberculosis
Issue Date: 2010
Publisher: Rajiv Mathur
Abstract: Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. Combination of techniques would, therefore, increase the sensitivity of diagnosis.
Peer Reviewed: yes
ISSN: 0975-1009
Document Type: Article
Appears in Collections:OLD - DCV - Trabalho Académico

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