Please use this identifier to cite or link to this item: http://hdl.handle.net/10348/2933
Title: Diagnosis of Mycobacterium avium complex in granulomatous lymphadenitis in slaughtered domestic pigs by histopathological and molecular methods
Authors: Miranda, Carla
Matos, Manuela
Pires, Maria dos Anjos
Correia-Neves, Margarida
Ribeiro, Paulo
Vieira-Pinto, Madalena
Coelho, Ana Cláudia
Keywords: histopathology
Mycobacterium avium complex
PCR
Pigs
Issue Date: 2012
Abstract: The aim of this study was to compare two diagnostic methods for the detection of Mycobacterium avium complex (MAC) infection in lymph nodes with granulomatous lymphadenitis from slaughtered domestic pigs. Fifty affected lymph nodes were collected from 50 pigs and examined microscopically and by polymerase chain reaction (PCR). Microscopically, granulomatous lesions were observed in 92% of the samples, consisting mostly of central necrosis (78%) with dystrophic calcification (46%) and associated with inflammatory infiltration by epithelioid giant cells, lymphocytes, neutrophils (92%), eosinophils (60%) and Langhans-type cells (70%). In 64% of the lesions, a capsule of connective tissue was found. Acid-fast bacilli were observed in all cases. PCR detected DNA from Mycobacterium spp. in 82% (41/50) of the lymph nodes. MAC was confirmed in 58% (24/41) and M. avium avium/silvaticum subspecies in 39% (16/41). The results of this study suggest that combined histopathology and PCR of lymph nodes are useful in the diagnosis of granulomatous lymphadenitis in slaughtered pigs.
Peer Reviewed: yes
URI: http://hdl.handle.net/10348/2933
ISSN: 00219975
metadata.dc.relation.publisherversion: doi: 10.1016/j.jcpa.2012.05.005
Document Type: Other
Appears in Collections:OLD - DCV - Trabalho Académico

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