Please use this identifier to cite or link to this item: http://hdl.handle.net/10348/4295
Title: DNA sampling from body swabs of terrestrial slugs (Gastropoda: Pulmonata): a simple and non-invasive method for molecular genetics approaches.
Authors: Morinha, Francisco
Travassos, Paulo
Carvalho, Diogo
Magalhães, Paula
Cabral, João Alexandre
Bastos, Estela
Keywords: Conservation genetics
Phylogenetics
Evolution
Populations
Geomalacus maculosus
Genetic
DNA
Geomalacus maculosus
PCR
Issue Date: 2014
Citation: Morinha F. el al, 2014
Abstract: Studies on molluscan conservation genetics, phylogenetics and evolution provide important data to access the diversity, populations’ structure and dispersal patterns of these organisms (Barker, 2001). Current environmental changes associated to anthropogenic pressures may have several negative ecological impacts on molluscs (e.g. van der Meij, Moolenbeek & Hoeksema, 2009; Cameron, Pokryszko & Martins, 2012), involving the reduction in their habitat suitability, which is particularly damaging for terrestrial slugs requirements of persistence (e.g. Severns, 2005; Kappes, 2006). The literature on slugs is greatly biased towards those that are agricultural pests (Barker, 2002), while there is little information about benign native species such as Geomalacus maculosus (Platts & Speight, 1988). Therefore, it is mandatory to develop multidisciplinary studies that integrate genetic data, biological and behaviour features to understand the adaptive, evolutionary and population dynamics of these species. The improvement of non-invasive DNA-based methodologies is essential to minimize the potential harmful effects of these approaches, particularly in studies of rare or endangered molluscs. Although most of methods to collect biological materials for DNA isolation in molluscs are intrusive (e.g.Winnepenninckx, Backeljau & De Wachter, 1993; Sokolov, 2000; Pereira et al., 2011), some protocols have been proposed based on non-invasive sources of DNA in terrestrial and marine snails and slugs (Kawai et al., 2004; Armbruster, Koller & Baur, 2005; Palmer, Styan & Shearman, 2008; Re´gnier et al., 2011). All these successful strategies were developed considering the DNA isolation from foot mucus and specific procedures for sample collection. Nevertheless, the sampling protocols are time-consuming and require the handling of the individuals. The development of simple and efficient protocols to improve these characteristics can significantly enhance its field applicability. Thus, the main goal of this study is to demonstrate and report a non-invasive, rapid, efficient and cost-effective method based on DNA isolation from body swabs of terrestrial slugs. In this perspective, the hypothesis under study is that slugs’ body surface epithelial cells provide an alternative good DNA source for genetic analysis.
Peer Reviewed: yes
URI: http://hdl.handle.net/10348/4295
Document Type: Article
Appears in Collections:DEBA - Artigo publicado em Revista Científica Indexada

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