Desarrollo de una nueva multiplex de Y-STRs para su aplicación en Antropología Molecular y Genética Forense
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2015-07-01
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Tanto la Antropología Molecular como la Genética Forense son ciencias relativamente recientes que basan la mayoría de sus estudios en análisis de polimorfismos genéticos. Unos de los más estudiados son los microsatélites del cromosoma Y (Y- STRs), al permitir predecir haplogrupos e incluso, algunos (RM Y-STRs), diferenciar entre varones emparentados. En este trabajo se ha realizado una selección de ocho marcadores Y-STRs (DYS570, DYS576, DYS481, DYS627, DYS460, DYS518, DYS449 y DYF387S1) que no son empleados de forma convencional hoy día. Tras obtener las secuencias de los primers de los marcadores seleccionados, se realizaron diversos experimentos para conseguir las condiciones óptimas de amplificación de los marcadores por separado y en conjunto como parte de una reacción multiplex. Posteriormente, y gracias a los resultados obtenidos, se realizará la optimización de la reacción multiplex desarrollada y la marcación de los primers con distintos fluorocromos. Por último, se realizará la secuenciación y el análisis para hacer posible la aplicación de la reacción multiplex desarrollada al estudio de individuos de Granada, Málaga y Almería.
Molecular Anthropology and Forensic Genetics are both relatively young fields of research, which base the majority of their studies on the analysis of genetic polymorphisms. Short tandem repeats on the Y-chromosome (Y-STRs) are one of the most studied polymorphisms as they can be used as haplotype predictors and, some of them (RM Y-STRs), can help to differentiate between paternally related males. The aim of this project was to optimize a technique to study Y-STR markers and its application in past migration and forensics. We studied a selection of eight Y-STR markers (DYS570, DYS576, DYS481, DYS627, DYS460, DYS518, DYS449 and DYF387S1) which are not commonly used nowadays. Once we obtained the primer sequences of the markers we had chosen, several experiments were carried out in order to attain the optimal conditions for amplifying each marker separately and together as a multiplex PCR. Afterwards, and thanks to the results obtained, we will carry out the optimization of the developed multiplex PCR and we will attach different fluorochrome-labels to the primers used. Finally, DNA sequencing and genotyping will be carried out in order to study the population of Granada, Málaga and Almería (South Spain).
Molecular Anthropology and Forensic Genetics are both relatively young fields of research, which base the majority of their studies on the analysis of genetic polymorphisms. Short tandem repeats on the Y-chromosome (Y-STRs) are one of the most studied polymorphisms as they can be used as haplotype predictors and, some of them (RM Y-STRs), can help to differentiate between paternally related males. The aim of this project was to optimize a technique to study Y-STR markers and its application in past migration and forensics. We studied a selection of eight Y-STR markers (DYS570, DYS576, DYS481, DYS627, DYS460, DYS518, DYS449 and DYF387S1) which are not commonly used nowadays. Once we obtained the primer sequences of the markers we had chosen, several experiments were carried out in order to attain the optimal conditions for amplifying each marker separately and together as a multiplex PCR. Afterwards, and thanks to the results obtained, we will carry out the optimization of the developed multiplex PCR and we will attach different fluorochrome-labels to the primers used. Finally, DNA sequencing and genotyping will be carried out in order to study the population of Granada, Málaga and Almería (South Spain).