Utilize este identificador para referenciar este registo: http://hdl.handle.net/10348/8753
Título: Genetics and expression of anthocyanin pathway genes in the major skinpigmented Portuguese cultivar ‘Vinhão’ developing berries
Autor: Ferreira, Vanessa
Castro, Isaura Alberta Oliveira De
Carrasco, David
Carnide, Olinda Pinto
Rosa Arroyo-García
Palavras-chave: Vinhão
Berry skin color
MYBA1/A2
Gene expression
Data: 11-Set-2018
Editora: Elsevier
Citação: Vanessa Ferreiraa,, Isaura Castro, David Carrasco, Olinda Pinto-Carnide, Rosa Arroyo-García (2019). Genetics and expression of anthocyanin pathway genes in the major skin pigmented Portuguese cultivar ‘Vinhão’ developing berries. Scientia Horticulturae 244: 88-93.
Resumo: Vinhão’ is an autochthonous Portuguese cultivar with an intense black-bluish skin color, highly appreciated due to this feature. This study aimed to give the first insights into the genetic background that may be responsible for the skin color properties of cv. ‘Vinhão’. For this purpose, the allelic composition of MYBA1 and MYBA2 genes was investigated, along with quantification of the expression levels of structural and regulatory genes involved in the anthocyanin biosynthetic pathway via qRT-PCR. The molecular characterization of MYBA1 and MYBA2 loci revealed that cv. ‘Vinhão’ is homozygous for the functional allele in both genes, corresponding to the most ancestral haplotype, which is consistent with the high colored phenotype that characterizes this cultivar. There were no differences in the DNA sequence of the MYBA1 promoter region between cv. ‘Vinhão’ and the grapevine reference genome Pinot Noir. The expression patterns of genes playing key functional roles in anthocyanin biosynthesis was analyzed in four developmental stages. The dynamics occurring throughout grape berry development revealed the involvement of these genes in the progression of key development events, mainly from veraison to mature berries. These findings provide the first molecular characterization focused on the skin color feature of cv. ‘Vinhão’ to improve our understanding of the genetics behind its intense skin pigmentation.
Revisão por Pares: yes
URI: http://hdl.handle.net/10348/8753
ISSN: 0304-4238
Tipo de Documento: Artigo
Aparece nas colecções:DGB - Artigo publicado em Revista Científica Indexada

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