Análises laboratoriais parasitológicas em Medicina Veterinária
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2018-11-16
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Esta dissertação é resultado de um estágio realizado no laboratório veterinário INNO, tendo envolvido análise e processamento de amostras fecais de canídeos e felídeos, para um estudo dos endoparasitas mais frequentes. Para análise e processamento das amostras foram escolhidas três técnicas, sendo elas: técnica da flutuação com recurso à centrifugação, para pesquisa de ovos de helmintes e coccídeas; técnica de Baermann para pesquisa de parasitas pulmonares; e técnica de ZiehlNeelsen modificada para pesquisa de Cryptosporidium spp. e Giardia spp. No total deste trabalho foram processadas 250 amostras provenientes de Portugal (continente e ilhas), sendo que 214 amostras foram analisadas através da técnica da flutuação, 28 amostras por Ziehl-Neelsen modificado e 8 amostras pela técnica de Baermann. Como a amostragem foi superior na técnica da flutuação, dividimos as amostras em dois grandes grupos, o primeiro grupo de amostras fecais de recolhas em apenas 1 dia e o segundo grupo de amostras fecais de recolhas em 3 dias consecutivos. Estas recolhas foram efetuadas pelos médicos veterinários ou pelos proprietários. No total das 250 amostras recolhidas 15,6% dos animais foram positivos. Estes resultados apontam para a necessidade de estudos mais aprofundados nesta temática, para melhor se estabelecer a importância dos parasitas dos animais de companhia em termos de clínica médico-veterinária e de saúde pública.
This thesis is the result of an internship in the INNO laboratory, with the purpose of analyzing and processing fecal matter samples from canine and feline specimens. These were done for a study of the most common endoparasites. For the analysis and processing of the samples, three base techniques were chosen: Willis method (simple fluctuation using a centrifugal machine), to look for helminths eggs and coccidias, Baermannn technique to look for pulmonary parasites and Ziehl-Neelsen modified to look for Giardia spp. and Cryptosporidium spp. In total, 250 samples were analyzed during this internship, coming from mainland Portugal and the insular islands. From these, 214 samples were processed using the Willis method, 28 using Ziehl-Neelsen and 8 using the Baermann technique. As the number of samples was higher for the Willis method, I divided the samples into two big groups, the first group of fecal samples was collected in 1 day only, and the second group of fecal matter was collected in 3 consecutive days. These collections were performed by either the veterinarians or the owners. In the grand total of 250 samples collected, about 15,6% of the animals were infected. These results, although inconclusive, point to the necessity of further and deeper research in this area to be able to stablish if the incidence of parasites in company animals can be considered dangerous to public health.
This thesis is the result of an internship in the INNO laboratory, with the purpose of analyzing and processing fecal matter samples from canine and feline specimens. These were done for a study of the most common endoparasites. For the analysis and processing of the samples, three base techniques were chosen: Willis method (simple fluctuation using a centrifugal machine), to look for helminths eggs and coccidias, Baermannn technique to look for pulmonary parasites and Ziehl-Neelsen modified to look for Giardia spp. and Cryptosporidium spp. In total, 250 samples were analyzed during this internship, coming from mainland Portugal and the insular islands. From these, 214 samples were processed using the Willis method, 28 using Ziehl-Neelsen and 8 using the Baermann technique. As the number of samples was higher for the Willis method, I divided the samples into two big groups, the first group of fecal samples was collected in 1 day only, and the second group of fecal matter was collected in 3 consecutive days. These collections were performed by either the veterinarians or the owners. In the grand total of 250 samples collected, about 15,6% of the animals were infected. These results, although inconclusive, point to the necessity of further and deeper research in this area to be able to stablish if the incidence of parasites in company animals can be considered dangerous to public health.
Descrição
Dissertação de Mestrado Integrado em Medicina Veterinária apresentada à universidade de Trás-os-Montes e Alto Douro
Palavras-chave
Parasitologia , endoparasitas , saúde pública , análises clínicas , medicina veterinária