Please use this identifier to cite or link to this item: http://hdl.handle.net/10348/9570
Title: Chitosan upregulates the genes of the ROS pathway and enhances the antioxidant potential of grape (Vitis vinifera L. ‘Touriga Franca’ and ’Tinto Cão’) tissues
Authors: Singh, Rupesh
Soares, Bruno
Goufo, Piebiep
Castro, Isaura Alberta Oliveira De
Cosme, Fernanda
Sintra, Ana Lucia Rebocho Lopes Pinto E
Inês, António Francisco Henrique
Oliveira, Ana Alexandra Ribeiro Coutinho De
Falco, Virgílio
Keywords: chitosan
elicitor
Vitis vinifera L.
antioxidant activity
secondary metabolites
anthocyanins
ROS pathway
polyphenols
tannins
Issue Date: 3-Nov-2019
Publisher: MDPI
Citation: Rupesh K. Singh, Bruno Soares, Piebiep Goufo, Isaura Castro, Fernanda Cosme, Ana L. Pinto-Sintra, António Inês, Ana A. Oliveira, Virgílio Falco (2019). Chitosan Upregulates the Genes of the ROS Pathway and Enhances the Antioxidant Potential of Grape (Vitis vinifera L. ‘Touriga Franca’ and ’Tinto Cão’) Tissues. Antioxidants 8, 525; doi:10.3390/antiox8110525.
Abstract: Chitosan is an environmentally-friendly active molecule that has been explored for numerous agricultural uses. Its use in crop protection is well-known, however, other properties, such as bioactivity, deserve attention. Moreover, the modes of actions of chitosan remain to be elucidated. The present study assessed the levels of total phenolic compounds, the antioxidant potential, and the expression of reactive oxygen species (ROS) scavenging genes in the berries (skins and seeds), leaves, cluster stems, and shoots upon chitosan application on two red grapevine varieties (Touriga Franca and Tinto Cão). The application of chitosan on the whole vine before and after veraison led to the increased levels of polyphenols, anthocyanins, and tannins in Tinto Cão berries, and polyphenols and tannins in Touriga Franca berries, respectively. CUPric Reducing Antioxidant Capacity (CUPRAC) and Ferric Reducing Antioxidant Power (FRAP) assays indicated an increase in the antioxidant potential of berries. With the exception of ascorbate peroxidase (APX), all the ROS pathway genes tested, i.e., iron-superoxide dismutase (Fe-SOD), copper-zinc-superoxide dismutase (Cu/Zn-SOD), catalase (CAT), glutathione reductase (GR), glutaredoxin (Grx), respiratory burst oxidase (Rboh), amine oxidase (AO), peroxidase (POD) and polyphenol oxidase (PPO), were found up-regulated in chitosan-treated berries. Results from the analyses of leaves, stems, and shoots revealed that chitosan not only induced the synthesis of phenolic compounds but also acted as a facilitator for the transfer of polyphenols from the leaves to the berries.
Peer Reviewed: yes
URI: http://hdl.handle.net/10348/9570
ISSN: 2076-3921
Document Type: Article
Appears in Collections:DGB - Artigo publicado em Revista Científica Indexada



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