The w/w+ somatic mutation and recombination test (SMART) of Drosophila melanogaster for detecting antigenotoxic activity

Ver registo simples
dc.contributor.authorGaivão, Isabel O Neill de Mascarenhaspt_PT
dc.contributor.authorFerreira, Joãopt_PT
dc.contributor.authorSierra, Luisa Maríapt_PT
dc.date.accessioned2021-09-07T10:22:00Z
dc.date.available2021-09-07T10:22:00Z
dc.date.issued2020-03-12
dc.descriptionOver the years, many investigations in DNA damage and DNA repair mechanisms were made, in vitro and in vivo, aiming to know our environment and thus identifying the harmful compounds to our genome, to our health, leading to preventive actions such as prohibiting the commercialization of certain drugs, construction materials, foods and drinks.pt_PT
dc.description.abstractGenotoxicological studies are emerging as fundamental for knowing the hazards to our genome, to our health. Drosophila melanogaster is one of the preferable organisms for toxicological research considering its metabolic similarities (viz. on dietary input, xenobiotic metabolizing system, antioxidant enzymes and DNA repair systems) to mammals. Accordingly, somatic mutation and recombination tests (SMARTs) of D. melanogaster are fast and low-cost in vivo assays that have shown solid results evaluating genotoxicity. The w/w+ SMART uses the white (w) gene as a recessive marker to monitor the presence of mutant ommatidia (eye units), indicating the occurrence of point mutations, deletions, mitotic recombination or/and nondisjunction. Additionally, several studies used SMARTs to assess antigenotoxicity, with some using the w/w+ SMART. We reviewed the state of the art of the w/w+ SMART used for antigenotoxicity analysis, focusing on published results, aiming to contribute to the conception of a reliable protocol in antigenotoxicity. As such, genotoxic agents with known action mechanisms, as streptonigrin (oxidative stress inducer), were used as a genotoxic insult for proving the antigenotoxic effects of natural substances (e.g. seaweeds), demonstrating the presence of antimutagens in their composition. These antigenotoxicity studies are crucial for promoting preventive measures against environmental genotoxics that affect humans daily.pt_PT
dc.description.sponsorshipThis work was supported by the project UIDB/CVT/00772/2020, which was supported by the Portuguese Science and Technology Foundation (FCT), and by the Gobierno del Principado de Asturias (Oviedo, Spain) through Plan de Ciencia, Tecnología e Innovación (PCTI), co-financed by FEDER funds (Ref. FC-GRUPINIDI/ 2018/000242) and by the Ministerio de Economia y Competitividad (MINECO) of Spain under the Project CTQ2016-80060-C2-1Rpt_PT
dc.identifier.citationGaivão et al (2020)pt_PT
dc.identifier.urihttp://hdl.handle.net/10348/10674
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherINTECHOPEN LIMITEDpt_PT
dc.relation.ispartofCECAV - Centro de Ciência Animal e Veterináriapt_PT
dc.relation.publisherversionhttps://www.intechopen.com/chapters/71344pt_PT
dc.rightsopen accesspt_PT
dc.subjectDNA damagept_PT
dc.subjectAntigenotoxicitypt_PT
dc.titleThe w/w+ somatic mutation and recombination test (SMART) of Drosophila melanogaster for detecting antigenotoxic activitypt_PT
dc.typebook partpt_PT
degois.publication.firstPage1pt_PT
degois.publication.lastPage20pt_PT
degois.publication.locationEngland and Walespt_PT
degois.publication.titleGenotoxicity and Mutagenicity - Mechanisms and Test Methodspt_PT
dspace.entity.typePublicationen
Ficheiros
Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
antigenotoxicity2020.pdf
Tamanho:
1.27 MB
Formato:
Adobe Portable Document Format
Descrição:
Ver/Abrir
Coleções
DGB - Capítulo ou Parte de Livro