Chloroplast and nuclear DNA studies in Iberian Peninsula endemic Silene scabriflora subspecies using cpSSR and ISSR markers: Genetic diversity and phylogenetic relationships

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dc.contributor.authorFerreira, Vanessapt_PT
dc.contributor.authorCastro, Isaura Alberta Oliveira Dept_PT
dc.contributor.authorRocha, Joãopt_PT
dc.contributor.authorCrespí, António L.pt_PT
dc.contributor.authorPinto-Carnide, Olindapt_PT
dc.contributor.authorAmich, Franciscopt_PT
dc.contributor.authorAlmeida, Rubimpt_PT
dc.contributor.authorCarnide, Valdemar Pedrosapt_PT
dc.date.accessioned2018-08-23T14:20:36Z
dc.date.available2018-08-23T14:20:36Z
dc.date.issued2015
dc.description.abstractThe Iberian Peninsula is considered to be a center of natural distribution and diversity for several species of flora. In this study, the genetic diversity and phylogenetic relationships of Silene scabriflora from the Iberian Peninsula were analyzed using ISSR and cpSSR markers. A total of 161 ISSR markers were produced, with a percentage of polymorphic loci of 99.4%. A high level of genetic differentiation among S. scabriflora subspecies (S. scabriflora spp. scabriflora, S. scabriflora spp. megacalycina, S. scabriflora spp. gallaecica and S. scabriflora spp. tuberculata) was observed (Gst ¼ 0.3685), which was illustrated by UPGMA dendrogram. Molecular results are in agreement with subspecies morphological characterization, particularly supporting the morphological similarities between S. scabriflora spp. scabriflora and S. scabriflora spp. megacalycina. Three of the five cpSSR loci analyzed were polymorphic and the two different alleles found in each polymorphic locus were combined in two different haplotypes. The results obtained in this study provide evidences for a long distance dispersion theory, Southern of Spain as the biodiversity center of S. scabriflora; for speciation, Southern of Spain as a refugia-within-refugia considering the speciation that occurs in S. scabriflora spp. scabriflora found in this region and the possibility of S. scabriflora spp. scabriflora as being ancestral of the species.pt_PT
dc.description.sponsorshipThis work was supported by the Fundação para a Ciência e Tecnologia (FCT) SFRH/BD/43167/2008 (to J.R.) and SFRH/BD/96400/2013 (to V.F.) grants.pt_PT
dc.identifier.issn0305-1978
dc.identifier.urihttp://hdl.handle.net/10348/8642
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.ispartofCITAB - Centro de Investigação e de Tecnologias Agro-Ambientais e Biológicaspt_PT
dc.rightsrestricted accesspt_PT
dc.subjectSilene scabriflorapt_PT
dc.subjectMolecular markerspt_PT
dc.subjectGenetic differentiationpt_PT
dc.subjectGeographic variationpt_PT
dc.subjectRefugiapt_PT
dc.titleChloroplast and nuclear DNA studies in Iberian Peninsula endemic Silene scabriflora subspecies using cpSSR and ISSR markers: Genetic diversity and phylogenetic relationshipspt_PT
dc.typejournal articlept_PT
degois.publication.firstPage312pt_PT
degois.publication.lastPage318pt_PT
degois.publication.titleBiochemical Systematics and Ecologypt_PT
degois.publication.volume61pt_PT
dspace.entity.typePublicationen
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