Phylogenetic identification of Aeromonas from pigs slaughtered for consumption in slaughterhouses at the North of Portugal

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dc.contributor.authorFontes, M. C.
dc.contributor.authorSaavedra, M. J.
dc.contributor.authorMartins, C.
dc.contributor.authorMartínez-Murcia, A.
dc.date.accessioned2013-10-10T09:53:29Z
dc.date.available2013-10-10T09:53:29Z
dc.date.issued2011
dc.description.abstractIn the present study, 710 isolates of Aeromonas spp. have been collected from pig carcasses, diaphragm muscle, faeces, dehairing equipment and water in slaughterhouses at the North of Portugal. The isolates were obtained from a total of 154 samples. All Presumptive Aeromonas isolates were subjected to ERIC-PCR analysis and those which presented a different pattern were taken and the species classified by gyrB gene sequencing. We have found the species A. hydrophila, A. salmonicida, A. bestiarum, A. caviae, A. media, A. veronii, A. allosaccharophila, A. simiae and A. aquariorum. To our knowledge, this extent of Aeromonas species diversity has not been previously described from meat or from the slaughter environment, perhaps due to the unreliability of available identification methods. A noticeable level of isolate redundancy (strains with identical gyrB sequence) from different samples collected in different dates was also obtained, indicating that only a few predominant strains of these species persist at the slaughter system. It is also important to emphasise the presence of Aeromonas species previously associated with illness in man.por
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e Tecnologia (PhD grants SFRH/BD/25415/2005).por
dc.identifier.citationFontes, M. C. et al, 2011, Int. J. Food Microbiol, 146, 118-122por
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/10348/2778
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.ispartofCECAV - Centro de Ciência Animal e Veterináriapor
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.ijfoodmicro.2011.02.010por
dc.rightsrestricted accesspor
dc.subjectAeromonaspor
dc.subjectPig carcassespor
dc.subjectgyrB sequencingpor
dc.subjectERIC-PCRpor
dc.titlePhylogenetic identification of Aeromonas from pigs slaughtered for consumption in slaughterhouses at the North of Portugalpor
dc.typejournal articlepor
degois.publication.firstPage118por
degois.publication.issue2por
degois.publication.lastPage122por
degois.publication.titleInternational Journal of Food Microbiologypor
degois.publication.volume146por
dspace.entity.typePublicationen
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