Molecular characterization of berry skin color reversion on grape somatic variants

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dc.contributor.authorFerreira, Vanessapt_PT
dc.contributor.authorCastro, Isaura Alberta Oliveira Dept_PT
dc.contributor.authorCarrasco, Davidpt_PT
dc.contributor.authorPinto-Carnide, Olindapt_PT
dc.contributor.authorArroyo-García, Rosapt_PT
dc.date.accessioned2018-08-23T14:48:19Z
dc.date.available2018-08-23T14:48:19Z
dc.date.issued2018
dc.description.abstractBACKGROUND: During grapevine domestication somatic variation has been used as a source of diversity for clonal selection. OBJECTIVE: This work provides additional information on the molecular mechanisms responsible for berry skin color reversion on a subset of somatic variants for berry skin color never investigated before. METHODS:The berry color locus and its surrounding genomic region were genetically characterized through a layer-specific approach, which has already been proven to be a successful method to decipher the molecular mechanisms responsible for color reversions on somatic variants. RESULTS: Deletions of different extent and positions were detected among less-pigmented/unpigmented variants derived from a pigmented wild-type. These deletions affected only the inner cell layer in the less pigmented variants and both cell layers in the unpigmented variants. Regarding the pigmented variants derived from an unpigmented wild-type, only one group was distinguished by the Gret1 retrotransposon partial excision from the VvMybA1 promoter. Moreover, within this latter group, VvMybA2 showed an important role regarding the phenotypic variation, through the recovery of the functional G allele. CONCLUSIONS: This investigation focused on the berry color locus using somatic variants for berry skin color, promotes a better understanding of the evolutionary events behind their origin and variability, opening an opportunity for their use in the genetic improvement of varieties.pt_PT
dc.description.sponsorshipThis work was supported by European Investment Funds through FEDER/COMPETE/POCI – Operational Competitiveness and Internationalization Programme, under Project POCI-01-0145-FEDER-006958, and National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UID/AGR/04033/2013. V. Ferreira is also indebted to FCT for her grant (SFRH/BD/96400/2013). Work in R. Arroyo-García lab is funded by the Project Grants RTA2014-00029-C03-01 and RTA2014-00016-C02-01. The authors would like to thank INIAV (Instituto Nacional de Investigação Agrária e Veterinária), INRA (Institut National de la Recherche Agronomique) and UTAD (University of Trás-os-Montes and Alto Douro) for kindly providing the plant material.pt_PT
dc.identifier.issn1878-5093
dc.identifier.urihttp://hdl.handle.net/10348/8652
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.ispartofCITAB - Centro de Investigação e de Tecnologias Agro-Ambientais e Biológicaspt_PT
dc.rightsrestricted accesspt_PT
dc.subjectBerry skin colorpt_PT
dc.subjectGrapevinept_PT
dc.subjectL1 and L2 cell layerspt_PT
dc.subjectMYB genespt_PT
dc.subjectSomatic mutationpt_PT
dc.subjectSSR markerspt_PT
dc.titleMolecular characterization of berry skin color reversion on grape somatic variantspt_PT
dc.typejournal articlept_PT
degois.publication.firstPage147pt_PT
degois.publication.issue3pt_PT
degois.publication.lastPage162pt_PT
degois.publication.titleJournal of Berry Researchpt_PT
degois.publication.volume8pt_PT
dspace.entity.typePublicationen
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