Temporal changes in Neutral Endopeptidase/CD10 immunoexpression in the cyclic and early pregnant canine endometrium

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dc.contributor.authorPayan-Carreira, Rita
dc.contributor.authorSantos, Celso
dc.contributor.authorMiranda, Sónia
dc.contributor.authorNeto Pereira, Rosa L.
dc.contributor.authorSantos, Dario
dc.contributor.authorPires, Maria dos Anjos
dc.date.accessioned2014-07-18T13:29:47Z
dc.date.available2014-07-18T13:29:47Z
dc.date.issued2014-07-18
dc.description.abstractCD10 is a multifunctional transmembrane neutral endopeptidase (NEP), considered to be a reliable marker of ectopic human endometrial stroma. Available information in NEP/CD10 protein expression in animal endometria is scarce. This study focused on the immunolocalization of NEP/CD10 in the canine uterus and on its temporal changes during the oestrous cycle and early pregnancy (days 11 to 23 post-LH surge) in healthy females. NEP/CD10 expression was found in the canine endometrial stroma in all stages of the oestrous cycle, showing cyclic differences both in intensity and in distribution pattern. A small population of negative stromal cells in subsurface position was also observed. This population shared some morphological characteristics with the human predecidual cells, which became positive in progesterone-associated stages of the cycle. In addition, positive immunolabelling was also observed in canine myometrial stroma. In early pregnancy, the basal glandular epithelia and the syncytium cords, remained negative to this marker,, contrasting with the trophoblast and the lacunar epithelium. A weak to moderate intensity of immunolabelling was observed in the decidual cells, while stromal immunolabelling was more intense at the delimitation of the syncytium cords. In conclusion, CD10 is consistently expressed in the canine endometrial stroma and myometrium, but not in endometrial epithelia. The characteristic pattern seen in early pregnancy also suggests a role for this molecule in the process of embryo invasion at implantation.por
dc.description.sponsorshipFCT através dos projetos PTDC/CVT/66587/2006 e PEst-OE/AGR/UI0772/2014por
dc.identifier.otherDOI: 10.1016/j.theriogenology.2014.06.019
dc.identifier.urihttp://hdl.handle.net/10348/3215
dc.language.isoengpor
dc.peerreviewedyespor
dc.relation.ispartofCECAV - Centro de Ciência Animal e Veterináriapor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0093691X14003148por
dc.rightsopen accesspor
dc.subjectNeutral endopeptidasepor
dc.subjectCD10por
dc.subjectendometrial stromapor
dc.subjectoestrous cyclepor
dc.subjectearly pregnancypor
dc.subjectimmunohistochemistrypor
dc.subjectfemale dogpor
dc.titleTemporal changes in Neutral Endopeptidase/CD10 immunoexpression in the cyclic and early pregnant canine endometriumpor
dc.typejournal articlepor
degois.publication.firstPageAvailable online 30 June 2014por
degois.publication.titleTheriogenologypor
dspace.entity.typePublicationen
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