The w/w+ somatic mutation and recombination test (SMART) of Drosophila melanogaster for detecting antigenotoxic activity
dc.contributor.author | Gaivão, Isabel O Neill de Mascarenhas | pt_PT |
dc.contributor.author | Ferreira, João | pt_PT |
dc.contributor.author | Sierra, Luisa María | pt_PT |
dc.date.accessioned | 2021-09-07T10:22:00Z | |
dc.date.available | 2021-09-07T10:22:00Z | |
dc.date.issued | 2020-03-12 | |
dc.description | Over the years, many investigations in DNA damage and DNA repair mechanisms were made, in vitro and in vivo, aiming to know our environment and thus identifying the harmful compounds to our genome, to our health, leading to preventive actions such as prohibiting the commercialization of certain drugs, construction materials, foods and drinks. | pt_PT |
dc.description.abstract | Genotoxicological studies are emerging as fundamental for knowing the hazards to our genome, to our health. Drosophila melanogaster is one of the preferable organisms for toxicological research considering its metabolic similarities (viz. on dietary input, xenobiotic metabolizing system, antioxidant enzymes and DNA repair systems) to mammals. Accordingly, somatic mutation and recombination tests (SMARTs) of D. melanogaster are fast and low-cost in vivo assays that have shown solid results evaluating genotoxicity. The w/w+ SMART uses the white (w) gene as a recessive marker to monitor the presence of mutant ommatidia (eye units), indicating the occurrence of point mutations, deletions, mitotic recombination or/and nondisjunction. Additionally, several studies used SMARTs to assess antigenotoxicity, with some using the w/w+ SMART. We reviewed the state of the art of the w/w+ SMART used for antigenotoxicity analysis, focusing on published results, aiming to contribute to the conception of a reliable protocol in antigenotoxicity. As such, genotoxic agents with known action mechanisms, as streptonigrin (oxidative stress inducer), were used as a genotoxic insult for proving the antigenotoxic effects of natural substances (e.g. seaweeds), demonstrating the presence of antimutagens in their composition. These antigenotoxicity studies are crucial for promoting preventive measures against environmental genotoxics that affect humans daily. | pt_PT |
dc.description.sponsorship | This work was supported by the project UIDB/CVT/00772/2020, which was supported by the Portuguese Science and Technology Foundation (FCT), and by the Gobierno del Principado de Asturias (Oviedo, Spain) through Plan de Ciencia, Tecnología e Innovación (PCTI), co-financed by FEDER funds (Ref. FC-GRUPINIDI/ 2018/000242) and by the Ministerio de Economia y Competitividad (MINECO) of Spain under the Project CTQ2016-80060-C2-1R | pt_PT |
dc.identifier.citation | Gaivão et al (2020) | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10348/10674 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | INTECHOPEN LIMITED | pt_PT |
dc.relation.ispartof | CECAV - Centro de Ciência Animal e Veterinária | pt_PT |
dc.relation.publisherversion | https://www.intechopen.com/chapters/71344 | pt_PT |
dc.rights | open access | pt_PT |
dc.subject | DNA damage | pt_PT |
dc.subject | Antigenotoxicity | pt_PT |
dc.title | The w/w+ somatic mutation and recombination test (SMART) of Drosophila melanogaster for detecting antigenotoxic activity | pt_PT |
dc.type | book part | pt_PT |
degois.publication.firstPage | 1 | pt_PT |
degois.publication.lastPage | 20 | pt_PT |
degois.publication.location | England and Wales | pt_PT |
degois.publication.title | Genotoxicity and Mutagenicity - Mechanisms and Test Methods | pt_PT |
dspace.entity.type | Publication | en |
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