Chloroplast genome diversity in Portuguese grapevine (Vitis vinifera L.) cultivars

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dc.contributor.authorCastro, Isaura
dc.contributor.authorPinto-Carnide, Olinda
dc.contributor.authorOrtiz, Jesús María
dc.contributor.authorMartín, Juan Pedro
dc.date.accessioned2013-10-10T10:00:19Z
dc.date.available2013-10-10T10:00:19Z
dc.date.issued2013
dc.description.abstractGrapevine chloroplast (cp) DNA diversity was analysed for the first time through amplification and digestion of fragments of the large single copy (LSC)region by polymerase chain reaction–restriction fragment length polymorphism methodology and also by amplification of three microsatellite loci, previously described as polymorphic in grapevine. Thirty-eight grapevine cultivars collected mainly in the North of Portugal, including some neglected cultivars, four international cultivars (Chasselas, Muscat of Alexandria, Muscat of Hamburg and Pinot) and Vitis riparia and Vitis rupestris, were used in this study with the main goal of finding out their pDNA diversity and compare the obtained results with previously published data on cultivars from other regions to ascertain their possible origin. Two different alleles were found in each of the three cpSSR loci. Allele variants of the three loci combined in a total of three different haplotypes (A, B and D). The most frequent haplotype, A, was previously reported as the most frequent in Iberian Peninsula and Occidental Europe. Haplotype B was unique to Rabigato, Muscat of Alexandria, V. riparia and V. rupestris. This haplotype was previously proposed to be an ancestral haplotype. Twenty-seven fragments of the LSC region of Vitis vinifera cpDNA were amplified and then digested with HinfI and TaqI restriction enzymes. Polymorphisms were found in the trnT-psbC (TC) and orf184-petA (OA) fragments. In the TC fragment, the olymorphism corresponds to a point mutation in a restriction site of TaqI and is only present in all cultivars with cpSSR haplotype D. In the OA fragment, a short deletion exclusive to the Rabigato cultivar was found. In this case, one sequence tagged sitebased marker was developed and will be very useful in future phylogenetic and fingerprinting studies in a broader number of cultivars and in wild grapevine populations. Inference about the progenitors of the Touriga Franca cultivar is done. The present work supports and completes its origin as a descendent of the female and male parents, Marufo and Touriga Nacional.por
dc.identifier.citationIsaura Castro, Olinda Pinto-Carnide, Jesús María Ortiz, Juan Pedro Martín. Chloroplast genome diversity in Portuguese grapevine (Vitis vinifera L.) cultivars. Molecular Biotechnology 54: 528-540.por
dc.identifier.issn1073-6085
dc.identifier.urihttp://hdl.handle.net/10348/2785
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherHumana Press Incpor
dc.relation.ispartofCGB - Centro de Genética e Biotecnologiapor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007/s12033-012-9593-9por
dc.rightsrestricted accesspor
dc.subjectcpDNA ; cpSSR; haplotypes; PCR-RFLP; Vitis vinifera L.por
dc.titleChloroplast genome diversity in Portuguese grapevine (Vitis vinifera L.) cultivarspor
dc.typejournal articlepor
degois.publication.firstPage528por
degois.publication.issue2por
degois.publication.lastPage540por
degois.publication.titleMolecular Biotechnologypor
degois.publication.volume54por
dspace.entity.typePublicationen
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